Plaque Assay

Overview
Quantifies infectious viral particles either by plaque formation or immune detection, depending on the virus type.

Principle

• Viruses are incubated with a susceptible cell monolayer.

• Plaque assay: visible lysis plaques are counted.

Applications

• Accurate viral titer determination (PFU or FFU/mL)

• Comparing viral replication efficiency

• Supporting vaccine and antiviral studies

Deliverables from MIV
?? Quantitative titers in PFU/mL or FFU/mL
?? Stained images of plaques?
?? Detailed calculation reports
?? Technical advice on result application