Neutralization Assay

Overview
Measures the ability of antibodies to block viral infection, serving as the gold standard for evaluating functional immunity.

Principle

• Serum samples are mixed with live or pseudoviruses.

• Mixtures are added to susceptible cells.

• Reduction in infectivity is quantified (IC?? or PRNT??).

Applications

• Vaccine efficacy testing

• Clinical immunity assessments

• Antibody therapeutic evaluation

Deliverables from MIV
?? Neutralization titers reported as IC?? or PRNT??
?? Graphical representation of neutralization curves
?? Detailed assay report with interpretation